Differences

This shows you the differences between two versions of the page.

--- keyence_sop [2021/04/27 17:41] – furst
+++ keyence_sop [2022/07/28 19:35] (current) – [Keyence Microscope Training SOP] haley
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 =====Keyence Microscope Training SOP=====
-Last edited: Furst (11/03/20)
+Last edited: Haley (7/28/22)
-Instructor:
+====Training Checklist:====
+  *Safety: do not leave lights on, do not crash objectives, wear gloves if replacing bulb
+  *Microscope Setup
+    *Changing objectives
+    *Tilting the head
+    *Changing lighting elements
+    *Moving/positioning the stage
+  *At the Computer
+    *Initialize the XY stage
+    *Software lighting and image quality
+    *Depth up
+    *Measure
+    *Stitching
+  *Post Imaging: replacing objective, turn off microscope light, replace original glass
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 ====Overview:====
   * This training provides an introduction to using and operating the Keyence microscope including:
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       - The knob on the left side of the microscope body can then be used to raise or lower the stage
       - Once the stage has been positioned, gently snug up the silver locking ring on the right side of the microscope to lock the position in place. DO NOT OVER TIGHTEN
+  - **Focusing the lens**
+    - The lens also moves in z and can be controlled by either the center dial on the control or the computer mouse wheel.
 ====At the Computer: ====
   - **Initialize the XY stage**