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keyence_sop [2020/11/12 18:30] – furstkeyence_sop [2022/07/28 19:35] (current) – [Keyence Microscope Training SOP] haley
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 =====Keyence Microscope Training SOP===== =====Keyence Microscope Training SOP=====
-Last edited: Furst (11/03/20)+Last edited: Haley (7/28/22)
  
-Instructor: +====Training Checklist:==== 
- +  *Safety: do not leave lights on, do not crash objectives, wear gloves if replacing bulb 
-Date: +  *Microscope Setup 
- +    *Changing objectives 
-{| style="" +    *Tilting the head 
-|+ Attendees: +    *Changing lighting elements 
-! +    *Moving/positioning the stage 
- +  *At the Computer 
-! +    *Initialize the XY stage 
-Name +    *Software lighting and image quality 
-! +    *Depth up 
-Group or Company +    *Measure 
-! +    *Stitching 
-Signature +  *Post Imaging: replacing objective, turn off microscope light, replace original glass
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 ====Overview:==== ====Overview:====
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       - The knob on the left side of the microscope body can then be used to raise or lower the stage       - The knob on the left side of the microscope body can then be used to raise or lower the stage
       - Once the stage has been positioned, gently snug up the silver locking ring on the right side of the microscope to lock the position in place. DO NOT OVER TIGHTEN       - Once the stage has been positioned, gently snug up the silver locking ring on the right side of the microscope to lock the position in place. DO NOT OVER TIGHTEN
- +  - **Focusing the lens** 
-  +    - The lens also moves in z and can be controlled by either the center dial on the control or the computer mouse wheel.
 ====At the Computer: ==== ====At the Computer: ====
   - **Initialize the XY stage**   - **Initialize the XY stage**
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       - Select "depth up" from the right hand side bar then "quick composition and 3D"       - Select "depth up" from the right hand side bar then "quick composition and 3D"
       - Focus the microscope slightly below the lowest point on the object \\ {{:tools:keyencemicroscope:focuso_on_bottom.jpg?400|}}       - Focus the microscope slightly below the lowest point on the object \\ {{:tools:keyencemicroscope:focuso_on_bottom.jpg?400|}}
-      - Click "3D Display" on the Keyence control pad and the microscope will scan up the object and create a 3D file which can be analyzed for depth. \\ {{:tools:keyencemicroscope:large_depth_of_field.jpg?400|}}{{:tools:keyencemicroscope:3d_depth_map.jpg?400|}}+      - Click "3D Display" on the Keyence control pad and the microscope will scan up the object and create a 3D file which can be analyzed for depth. \\ {{:tools:keyencemicroscope:large_depth_of_field.jpg?400|}}{{:tools:keyencemicroscope:depth.png?400|}}
   - **Measure**   - **Measure**
     - Measurements can be taken by selecting the "Measure" tab to measure the current view of the microscope or after a Depth up scan by selecting the "measure" tab after scan is complete     - Measurements can be taken by selecting the "Measure" tab to measure the current view of the microscope or after a Depth up scan by selecting the "measure" tab after scan is complete
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       - select "stitch around the current position"       - select "stitch around the current position"
       - click "Start stitching" and press enter to stop the stitching whenever the image collected reaches the desired size.       - click "Start stitching" and press enter to stop the stitching whenever the image collected reaches the desired size.
-    - Stitch after specifying the area \\ {{:tools:keyencemicroscope:stitch_set_bounds.jpg?400|}}+    - Stitch after specifying the area \\ {{:tools:keyencemicroscope:stitch_set_bounds.png?400|}}
       - Click select range       - Click select range
       - Position the microscope as the minimum x position and select left, then maximum x position and select right       - Position the microscope as the minimum x position and select left, then maximum x position and select right
keyence_sop.1605205816.txt.gz · Last modified: 2020/11/12 18:30 by furst