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keyence_sop [2020/11/12 18:19] – furstkeyence_sop [2022/07/28 19:35] (current) – [Keyence Microscope Training SOP] haley
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 =====Keyence Microscope Training SOP===== =====Keyence Microscope Training SOP=====
-Last edited: Furst (11/03/20)+Last edited: Haley (7/28/22)
  
-Instructor: +====Training Checklist:==== 
- +  *Safety: do not leave lights on, do not crash objectives, wear gloves if replacing bulb 
-Date: +  *Microscope Setup 
- +    *Changing objectives 
-{| style="" +    *Tilting the head 
-|+ Attendees: +    *Changing lighting elements 
-! +    *Moving/positioning the stage 
- +  *At the Computer 
-! +    *Initialize the XY stage 
-Name +    *Software lighting and image quality 
-! +    *Depth up 
-Group or Company +    *Measure 
-! +    *Stitching 
-Signature +  *Post Imaging: replacing objective, turn off microscope light, replace original glass
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 ====Overview:==== ====Overview:====
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       - Lock the head in its tilted position using the large tilt lock knob. Make sure knob is snug before letting go of the head but do not overtighten       - Lock the head in its tilted position using the large tilt lock knob. Make sure knob is snug before letting go of the head but do not overtighten
       - If desired angle is greater then 60 degrees, pull the locking pin out away from the microscope before retightening the tilt lock knob. This should allow the microscope head to tilt up to 90 degrees. Be incredibly careful as the stage and objective will become very close at angles above 60 degrees.       - If desired angle is greater then 60 degrees, pull the locking pin out away from the microscope before retightening the tilt lock knob. This should allow the microscope head to tilt up to 90 degrees. Be incredibly careful as the stage and objective will become very close at angles above 60 degrees.
-  - **Changing lighting elements** \\ {{:tools:keyencemicroscope:attatchments.jpg?400|}}+  - **Changing lighting elements** \\ {{:tools:keyencemicroscope:attatchments.png?400|}}
     - Switching to fiberoptic Epi-illumination for 100-1000x objective     - Switching to fiberoptic Epi-illumination for 100-1000x objective
       - With the 100-1000x objective installed turn on the MI-150 fiberoptic lamp       - With the 100-1000x objective installed turn on the MI-150 fiberoptic lamp
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       - The knob on the left side of the microscope body can then be used to raise or lower the stage       - The knob on the left side of the microscope body can then be used to raise or lower the stage
       - Once the stage has been positioned, gently snug up the silver locking ring on the right side of the microscope to lock the position in place. DO NOT OVER TIGHTEN       - Once the stage has been positioned, gently snug up the silver locking ring on the right side of the microscope to lock the position in place. DO NOT OVER TIGHTEN
- +  - **Focusing the lens** 
-  +    - The lens also moves in z and can be controlled by either the center dial on the control or the computer mouse wheel.
 ====At the Computer: ==== ====At the Computer: ====
   - **Initialize the XY stage**   - **Initialize the XY stage**
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   - **Software lighting and Image Quality**   - **Software lighting and Image Quality**
     - Within Software lighting two tabs are available:     - Within Software lighting two tabs are available:
-      - Brightness \\ {{:tools:keyencemicroscope:lighting.jpg?400|}}+      - Brightness \\ {{:tools:keyencemicroscope:lighting.png?400|}}
         - Adjust shutter speed and camera settings (set to auto)         - Adjust shutter speed and camera settings (set to auto)
-      - Lighting \\ {{:tools:keyencemicroscope:other_lighting.jpg?400|}}+      - Lighting \\ {{:tools:keyencemicroscope:other_lighting.png?400|}}
         - Epi-illumination         - Epi-illumination
           - Adjust/turn on internal objective illumination (press "light" on console to toggle)           - Adjust/turn on internal objective illumination (press "light" on console to toggle)
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       - Select "depth up" from the right hand side bar then "quick composition and 3D"       - Select "depth up" from the right hand side bar then "quick composition and 3D"
       - Focus the microscope slightly below the lowest point on the object \\ {{:tools:keyencemicroscope:focuso_on_bottom.jpg?400|}}       - Focus the microscope slightly below the lowest point on the object \\ {{:tools:keyencemicroscope:focuso_on_bottom.jpg?400|}}
-      - Click "3D Display" on the Keyence control pad and the microscope will scan up the object and create a 3D file which can be analyzed for depth. \\ {{:tools:keyencemicroscope:large_depth_of_field.jpg?400|}}{{:tools:keyencemicroscope:3d_depth_map.jpg?400|}}+      - Click "3D Display" on the Keyence control pad and the microscope will scan up the object and create a 3D file which can be analyzed for depth. \\ {{:tools:keyencemicroscope:large_depth_of_field.jpg?400|}}{{:tools:keyencemicroscope:depth.png?400|}}
   - **Measure**   - **Measure**
     - Measurements can be taken by selecting the "Measure" tab to measure the current view of the microscope or after a Depth up scan by selecting the "measure" tab after scan is complete     - Measurements can be taken by selecting the "Measure" tab to measure the current view of the microscope or after a Depth up scan by selecting the "measure" tab after scan is complete
-      - Select the desired tool and desired points to measure from on the sample. The microscope will automatically display the length of lines or radius of arcs/circles drawn \\ {{:tools:keyencemicroscope:measuring.jpg?400|}}+      - Select the desired tool and desired points to measure from on the sample. The microscope will automatically display the length of lines or radius of arcs/circles drawn \\ {{:tools:keyencemicroscope:measuring.png?400|}}
  
   - **Stitching**   - **Stitching**
-    - Center surround stitching \\ {{:tools:keyencemicroscope:stitch.jpg?400|}}+    - Center surround stitching \\ {{:tools:keyencemicroscope:stitch.png?400|}}
       - Center the sample in the frame and select "stitching" from the sidebar        - Center the sample in the frame and select "stitching" from the sidebar 
       - select "stitch around the current position"       - select "stitch around the current position"
       - click "Start stitching" and press enter to stop the stitching whenever the image collected reaches the desired size.       - click "Start stitching" and press enter to stop the stitching whenever the image collected reaches the desired size.
-    - Stitch after specifying the area \\ {{:tools:keyencemicroscope:stitch_set_bounds.jpg?400|}}+    - Stitch after specifying the area \\ {{:tools:keyencemicroscope:stitch_set_bounds.png?400|}}
       - Click select range       - Click select range
       - Position the microscope as the minimum x position and select left, then maximum x position and select right       - Position the microscope as the minimum x position and select left, then maximum x position and select right
keyence_sop.1605205140.txt.gz · Last modified: 2020/11/12 18:19 by furst